The Effect of Melatonin on Gene Expression and Activity of the Sirt1 a
The Effect of Melatonin on Gene Expression and Activity of the Sirt1 and Its Target Genes Catalase and MnSOD in Multiple Sclerosis Patients and Healthy Subjects
What it found
We found no results in the registry or in papers.
This trial finished 11 years ago. We found no results in the registry or in any paper that cites it.
What was tested
Treatment tested
- Melatonin
Melatonin receptor agonist
- Hydrogen peroxide
What it measured
Main measures
Relative gene expression by real-time PCR (polymerase chain reaction)
Change from baseline after 12-hour treatment with melatonin and 2 -hours treatment with H2O2
Immune response
Other measures (3)
MnSOD enzyme activity assay
Change from baseline after 12-hour treatment with melatonin and 2 -hours treatment with H2O2
Catalse enzyme activity assay
Change from baseline after 12-hour treatment with melatonin and 2 -hours treatment with H2O2
SIRT1 enzyme activity assay
Change from baseline after 12-hour treatment with melatonin and 2 -hours treatment with H2O2
Who could take part
Relapsing MS · 20 to 40
Show the full criteria
Inclusion criteria
patients with relapsing-remitting multiple sclerosis. The diagnosis of MS was based on the McDonald criteria . All patients were newly diagnosed cases with an Expanded Disability Status Scale (EDSS) score ≤ 6 and had never received immunomodulatory and immunosuppressive drugs. -
Exclusion criteria
* The exclusion criteria were as follows: 1) treated with antioxidant supplements, anti-inflammatory drugs, and vitamins in the previous 6 months; 2) had a history of acute or chronic infection, malignancy, diabetes (type I and II), and any clinically significant systemic disease; 3) was currently pregnant or lactating; 4) was currently smoking; 5) used contraceptive medication during the previous 6 months.
As registered. Criteria can change, so ask the trial team or your neurologist.
About this trial
In the sponsor's words
The purpose of this study is to investigate the gene expression and activity of SIRT1, and forkhead box O3 pathway by means of investigating target genes MnSOD and catalase in multiple sclerosis and healthy subjects. Also, we investigate the effect of melatonin on interaction between SIRT1 activity and target genes MnSOD and catalase in multiple sclerosis and healthy subject .Additionally, we evaluate effect the probable effect of melatonin treatment on gene expression and activity of above-mentioned genes in peripheral blood mononuclear cells of healthy subjects-treated with H2O2.
Papers
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History
Started
Sept 2012
Main results due
Jun 2015
Finished
Sept 2015